Primary dissolution of compounds, thawing, and resolution of precipitated compounds is a use of AFA™ that is gaining wide acceptance in the compound management community.

Current dissolution technologies are either inadequate or have significant drawbacks compared to AFA. This is illustrated in the table below:

Feature	Benefit	Technique
		AFA	Vortex	Sonication
Isothermal	No compound degradation	Yes	Yes	No
Non-contact	Decapping not necessary Washing of instrument not necessary No instrument or compound cross contamination	Yes	Yes	No
Controllable Mechanical Energy	Fast & highly efficient Capable of gum / glue / "glass" dissolution Fast	Yes	No	No/Yes
Automation friendly	Easy integration	Yes	No	No
No consumable	Reduced cost	Yes	Yes	No
Capable of thawing individual wells (3s for a 2D tube)	Reduced freeze thaw cycle for plates	Yes	No	No
Plate / tube independent	Flexible	Yes	No	No

To date a large amount of data has been generated on the application of AFA in compound dissolution. We have included a video to illustrate this; however data is also presented below in comparison to a microplate Sonicator. The data was analyzed using quantitative liquid chromatography.

Clozapine dissolution carried out in a polypropylene 96 well Plate in DMSO