DNA Shearing
One of the unique features of the Covaris AFA™ is the ability to shear DNA. Double stranded DNA will fragment when exposed to the energy of AFA. This shearing is site independent and is a perfect substrate for the production of DNA libraries.
The system is computer controlled and once the conditions have been optimized for the size range you require it is highly repeatable. In addition to this, AFA allows for a large number of samples to be processed in a short period of time with no washing between samples.
The Covaris systems are becoming a "Must-have" for the Next-gen sequencing tasks, because:
- Isothermal processing—high recovery
- Non contact, in closed vessel—no chance of cross contamination
- Very little starting material required (1ng of genomic DNA required for Illumina sequencer)
- Compatible & validated with ALL Next-gen sequencing platforms—SOLiD, Roche/454, Illumina...
- Possible for amplification-free library construction
- Reproducibly shear PCR products to smaller fragments
- Recommended by Agilent SureSelect™ Target Enrichment System
- Tight size distribution and highly reproducible
- Automatable for higher throughout (i.e. 24 or 96 sample format)
- Fast and easy to use
Protocol—Latest for DNA Shearing
Latest data from customers
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