DNA Shearing with AFA
100bp to 5kb
Double stranded DNA will fragment when exposed to the energy of AFA. This shearing is site-independent and is a perfect substrate for the production of DNA libraries which is why the WORLD'S LEADING GENOME CENTERS use Covaris systems.
Samples are exposed to AFA under highly controlled conditions such that fragment sizes can be precisely selected and, when run under the same conditions, results are HIGHLY REPRODUCIBLE. Altering conditions enables you to select fragment sizes from SMALL TO LARGE (100bp-5kb) with remarkable PRECISION. In addition AFA is NON-CONTACT and processes sample under ISOTHERMAL conditions ensuring the highest quality fragments for Next-Gen sequencing.
The Covaris systems are considered the "Gold-Standard" in leading Genome Centers because:
- Covaris ELIMINATES THE RISK OF G/C BIAS, common with enzymatic shearing.
- Results are HIGHLY REPRODUCIBLE; run-to-run, user to user, day to day you will get consistent DNA
- VERSATILITY: a single system can be used for fragment size selection from 100bp to 5kb
- The technology yields PRECISE and tight fragment size distribution
- It is ISOTHERMAL, leading to high recovery and high fidelity of your DNA
- The system is FAST, EASY TO USE and automatable for higher throughput
Covaris systems are recommended by the WORLD'S LEADING MANUFACTURERS of Next-Gen Sequencers and related technologies (LIFE TECHNOLOGIES, ILLUMINA, AGILENT) and the WORLD'S LEADING GENOME CENTERS. Contact Us now to learn more about how our technology can enhance your Next-Gen sequencing results.
 
 DNA Shearing with g-TUBE
6kb to 20kb
g-TUBE, the latest innovation from Covaris, is a single-use consumable that enables scientists to shear genomic DNA into selected fragments sizes ranging from 6kbp to 20kbp. The only equipment needed is a compatible bench-top centrifuge and a pipette.
g-TUBE uses centrifugal force (the "g" in g-TUBE) to push the sample through a precisely manufactured orifice in the embedded ruby. This produces shearing forces in the sample that fragment the DNA. Fragment size is selected by adjusting the centrifuge rotor speed which alters the flow rate through the ruby and thus the shearing forces. Higher centrifugation RPM will produce shorter fragments and the protocol provide by Covaris contains the settings needed to select a fragment size between 6kbp and 20kbp.
Remarkably, the entire process takes only 3 minutes and up to 24 samples can be processed simultaneously.
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