Plasma Extraction
Plasma: This standard procedure calls for extraction with acetonitrile (3 times 3:1 acetonitrile:sample). The acetonitrile crashes out plasma proteins after the first addition, giving a very gummy protein pellet that is difficult to break-up for further extractions.
Current protocol 3:1 acetonitrile, vortex spatula.
By using Covaris acoustics, it is possible to greatly improve upon the traditional methods of plasma extraction. By reducing the ratio of Acetonitrile/Plasma to 1:1, then acoustically mixing, this creates a very homogenous flocculent prep. Then 2 parts of acetonitrile are added for the final extraction.
It is also possible to spin down the pellet, cryo-pulverize it to rapidly shatter the pellet, and then carry out extraction on the increased surface area.
By carrying out the extraction in two parts there is NO gummy material. The final constituency is very flocculent, with a large surface area allowing highly efficient repeatable extractions.
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1&2
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1:1 acetonitrile initial acoustic mix followed by 3:1 acetonitrile extraction. Very flocculent end, large surface area for extraction.
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3
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Cryo-pulverized using CryoPrep: standard prep spun down, pellet fractured, then acoustic mixing, and settled
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Unique Features & Benefits
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Benefit |
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Non-contact
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Sample is not contaminated
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Controllable mechanical energy
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Dissolution is controllable for all application areas
Increased biological activity in preparations
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Electronic driven
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Highly repeatable
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Isothermal
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No heat generated
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Fast
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Many samples can be processed in very short time frames
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Samples are closed tube
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Safe - no aerosol risk
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Scalable
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Volumes from 200µl - 10mls
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Automation enabled
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E-Series can process microplates or racks of tubes
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