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DNA Shearing
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Wellcome Trust Sanger Center
Article on Nature Methods, using Covaris AFA technology to shearing DNA:
Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes (web link)
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Wellcome Trust Sanger Center
Article in Nature Methods 2008 using the AFA technology to fragment DNA samples for their sequencing protocols
Nature Methods AFA technology (web link)
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US Department of Energy Joint Genome Institute
Poster presented at 4th Sequencing, Finishing, Analysis in the Future (SFAF) conference:
Cloning and Sequencing with Trace Amount of DNA on Roche/454 and Illumina Platforms (PDF/388k)
The poster demonstrated improved protocol for library construction from trace amount of starting material (as low as 1ng of genomic DNA).
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Invitrogen/Life Technologies
Poster using Covaris S2 to shearing DNA for research on:
Selective enrichment and massively parallel sequencing of medically relevant genomic regions using long-range PCR (PDF/130kb)
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Roche/454
Poster presented at 2009 Plant and Animal Genome XVII Conference
Automation of the GS FLX Standard Library Preparation and Implementation at the 454 Sequencing Center (PDF/1.1mb)
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Broad Institute
Poster presented at Marco Island AGBT 2008 meeting for use of Covaris for DNA shearing sample preparation for sequencing.
Broad AGBT 2008 Poster (PDF/188kb)
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Roche/454
Posters on Automated Library Preparation (PDF/168kb)
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Broad Institute
Poster presented at AGBT 2006 for use of Covaris AFA to improve DNA shearing methodology
Broad AGBT 2006 Poster (PDF/105kb)
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Purdue University and Fred Hutchinson Cancer Research Center
The Maize TILLING Project--Updates and EcoTILLING Industrial Germplasm
Poster on DNA shearing using Covaris E, in preparation of sequencing with AB SOLiD
Maize TILLING Poster (PDF/888kb)
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Washington University
Article in PLoS ONE Journal, December 2008, using Covaris S2 instrument for DNA shearing
Transcriptome-Wide Identification of Novel Imprinted Genes in Neonatal Mouse Brain (web link)
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Broad Institute
DNA shearing using Covaris S2: Ab initio construction of a eukaryotic transcriptome by massively parallel mRNA sequencing (PDF/777kb)
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Covaris
Poster presented at Sequencing, Finishing and Analysis in the Future (SFAF) 2009: Chromatin Shearing Based on Covaris Adaptive Focused Acoustics (AFA) (PDF/1.2mb)
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Wellcome Trust Sanger Center and Centre National de Génotypage
Presentations from WTSI (click for presentation PDF/1.5mb) and CNG (click for presentation PDF/4.2mb) during Illumina user meeting in Crete, April 2009, DNA shearing using Covaris E-series, in preparation Illumina next-gen sequencing platform
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Princeton University
Article on BioMed Central, using Covaris instrument to shearing DNA, in preparation for Illumina sequencer: Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays
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DNA/RNA Extraction
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The Cancer Genome Atlas Research Network
Effective DNA/RNA extraction after tissue disruption using Covaris AFA:
Nature article on October 2008 (Vol. 455, 1061-1068, doi:10.1038/nature07385):
Comprehensive genomic characterization defines human glioblastoma genes and core pathways (PDF/449k)
Protocol on P. 14 in the Nature Supplementary Information (PDF/3mb).
Biospecimens were provided by the tissue source sites and processed by the Biospecimen Core Resource
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Covaris
Poster presented at American Association for Cancer Research (AACR) 2009:
Novel technology used in cancer sample preparation (PDF/619k)
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Journal of Clinical Virology
Article appearing in the Journal of Clinical Virology:
Development of a real-time multiplex RSV detection assay for difficult respiratory samples, using ultrasone waves and MNAzyme technology (PDF/332k)
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Florida Gulf Coast University
Presentation for the American Academy of Sciences, February 2007, which describes the method of extraction of DNA from bone using Covaris AFA technology
Forensics Samples Proceedings (PDF/19kb)
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Rosetta Inpharmatics LLC/Merck & Co
Poster presented at CHI Sample Prep 2004 for use of Covaris AFA in tissue extraction
CHI Sample Prep 2004 Poster (PDF/1.07mb)
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Tissue Disruption/Homogenization
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WIRED Article
This article discusses how outdated tissue collection and preparation methodologies can have a significant negative impact on scientific research and potential medical breakthroughs.
WIRED Article (PDF/3.8mb)
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GlaxoSmithKline
Presentation at Screening, MedChem and ADMET Europe, 2009:
Fully-automated sample preparation and purification of homogenized tissue samples
GlaxoSmithKline 2009 Presentation (PDF/4.5mb)
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Solvay Pharmaceuticals 2008
Study comparison of Covaris AFA-based extraction versus combustion techniques with radio-tagged samples. Presented at the SMI 3rd annual ADMET conference, 2 & 3 July, 2008, London, UK
Solvay Pharma 2008 Poster (PDF/142kb)
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BG Medicine
Covaris E-series instrument was used to disrupt and homogenize tissue to extract protein.
Article on Mol. BioSyst., 2008, 4, 249 – 259
Correlation network analysis for data integration and biomarker selection (PDF/879kb)
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GlaxoSmithKline
Poster presented at the 2009, 16th North American Regional Meeting, International Society for the Study of Xenobiotics (ISSX), Baltimore, Maryland, USA.
Efficient Recoveries of Drug-Related Material from Rodent Eye Tissues for Metabolite Analysis Using Novel Adaptive Focused Acoustic Technology (PDF/97kb)
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Tissue Disruption/Homogenization, compound extracted from tissue
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HUPO
Poster presented at the 8th Annual HUPO World Congress in Toronto, Canada on September 26-30th, 2009. (PDF/8mb)
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Millennium Pharmaceuticals
Poster presented at ASMS 2004 for use of Covaris AFA in tissue extraction
Millennium ASMS Poster (PDF/90kb)
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Johnson & Johnson Pharmaceutical R&D
Poster presented at 2006 ASMS Conference on Mass Spectrometry for use of Covaris AFA for tissue homogenization prior to bioanalysis
ASMS 2006 Poster (PDF/97kb)
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Ferring Pharmaceuticals 2008
Poster presented by Ferring Pharmaceuticals, on tissue freeze-fracture Covaris CryoPrep system with extraction by acoustic homogenization with Covaris S2
Ferring Pharma 2008 Poster (PDF/211kb)
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Charles River Laboratories
Poster presented at AAPS 2006 for use of Covaris AFA to homogenize Peripheral Blood Mononuclear Cells (PBMCs)
Charles River Labs 2006 Poster (PDF/183kb)
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Amgen 2007
Poster presented at the 2007 ASMS for a method of tissue analysis using homogenization with Covaris AFA technology
ASMS Amgen 2007 Poster (PDF/411kb)
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Amgen 2006
Poster presented at the 2006 ASMS for a method for the determination of free drug in mouse brain by acoustic homogenization, ultracentrifugation and LC-MS/MS
ASMS Amgen 2006 Poster (PDF/119kb)
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United States Army 2008
Poster presented at 2008 American Society for Mass Spectrometry, Denver, Colorado to extract small molecules from skin tissue
U.S. Army ASMS Poster (PDF/4.0mb)
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Tissue Disruption/Homogenization, plant protein extracted
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National Institute of Crop Science, Japan
Plasma membrane proteome in Arabidopsis and rice (PDF/226kb)
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National Institute of Crop Science, Japan, 2008
Acoustic Technology for High-Performance Disruption and Extraction of Plant Proteins
National Institute of Crop Science (web link)
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Tissue Disruption/Homogenization, proteasome extracted
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Millennium Pharmaceuticals
Article in Molecular Cancer Therapeutics, December 2006, Covaris E-series instrument used in sample preparation for proteasome enzymatic assays.
Comparison of biochemical and biological effects of ML858 (salinosporamide A) and bortezomib (PDF/944kb)
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BG Medicine, Inc; National Center for Toxicological Research, US FDA
Article in Toxicologic Pathology ( Vol 37; pages 52-64), 2009, Covaris CryoPrep Tissue Tubes were used to pulverized frozen liver lobes and E-series instrument used to extract proteins for proteomic analysis.
The Liver Toxicity Biomarker Study: Phase I Design and Preliminary Results
(PDF/356kb)
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Cell Lysis
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School of Chemistry and Manchester Center for Integrative System Biology, University of Manchester, UK and Laboratory of Bacteriology, Rega Institute, Katholieke Universiteit Leuven, Belgium
Article in Analyst (Vol 135, pages 934-942, 2010) compares use of Covaris S instrument with manual vortex/freeze-thaw method of extraction metabolites from Streptomyces lividans TK24. More efficient recovery of metabolites was achieved using AFA extraction.
Article in Analyst (PDF/400kb)
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Merck & Co.
Article presented on Biotechnology Progress (Vol. 24, Issue 3, page 606-614), using Covaris AFA technology was optimized for the disruption of milligram quantities of yeast cells for the subsequent purification of recombinant human papillomavirus (HPV) virus-like particles (VLPs).
Bioseparations and Downstream Processing: A Microscale Yeast Cell Disruption Technique for Integrated Process Development Strategies (web link)
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GlaxoSmithKline Pharmaceuticals
Oral presentation at European Laboratory Robotics Information Group meeting in Cambridge, England April 27, 2006 on using Covaris process for cell lysis (E.coli)
GSK ELRIG 2006 Presentation (PDF/1.52mb)
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Johns Hopkins University and SpectralWorks
Article presented at ASMS 2004, drug extracted from red blood cells using Covaris E-series instrument: A metabolomics study of Plasmodium falciparum infection of red blood cells in the absence and presence of antimalarials
Metabolomics Study Presentation (PDF/143kb)
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Pharmaceutical Research/Compound Formulation
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Southern Research Institute
Southern Research Institute Poster presented at 2009 Association of Laboratory Automation Annual Conference:
Acoustic Droplet Ejection—New Liquid Handling Technology, New Challenges in Automation and Implementation into the Work Flow
ALA 2009 Poster (PDF/563kb)
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Boehringer Ingelheim
Poster presented at 2008 AAPS Annual Meeting and Exposition: Application of Covaris AFA System for the Preparation of Suspensions for Early PK Studies
Poster web link
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GlaxoSmithKline Pharmaceuticals
Poster presented at 2007 DMDG for use of Covaris AFA technology in Psychiatry CEED DMPK
GSK DMDG 2007 Poster (PDF/680kb)
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Covaris AFA Ultra Sonication Benefits
Covaris AFA Ultra Sonication Benefits 2007 poster illustrating the benefits of Covaris AFA ultra sonication on the HTS process from compound master to HTS assay plate
Covaris AFA Ultra Poster (PDF/63kb)
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Pharmaceutical Research/Sample Preparation
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Bayer CropScience
Article published about using the Covaris AFA technology for HPLC Sample Preparation. Published on the web by ACS Publications, ASAP Journal of Combinatorial Chemistry, ASAP Article, 10.1021/cc800095v, September 13, 2008
Bayer CropScience (web link)
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Pharmaceutical Research/Compound Management
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GlaxoSmithKline Pharmaceuticals
Poster presented at SBS 2005 on implementation of Covaris AFA throughout the Drug Discovery pipeline
GSK 2005 Poster (PDF/680kb)
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GlaxoSmithKline Pharmaceuticals
Poster presented at LabAutomation 2006 for use of Covaris AFA in Compound Management applications
GSK LabAuto 2006 Poster (PDF/811kb)
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GlaxoSmithKline Pharmaceuticals
Oral presentation at Miptec meeting in Basel, Switzerland 2006 using the Covaris technology for applications in compound management, chemistry and high-throughput screening
GSK Miptec 2006 Presentation (PDF/453kb)
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GlaxoSmithKline Pharmaceuticals
Presentation given at the 2006 Society for Biomolecular Sciences (SBS) Sample Management Special Interest Group comparing Covaris AFA technology vs. conventional sonication for compound management applications
Covaris SBS 2006 Presentation (PDF/890kb)
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GlaxoSmithKline Pharmaceuticals
Article published in the Journal of Biomolecular Screening evaluating several Covaris Adaptive Focused Acoustic systems and some of their applications to Compound Management.
GSK 2008 JBS article (web link)
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